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Ramtin Vamenani

Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari, Mazandaran

Introduction

In 2011, I received my B.Sc.degree in plant protection at the Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran. Right after graduation, I commenced my M.Sc. career in the field of Plant Pathology at the Department of Plant Pathology at Sari Agricultural Sciences and Natural Resources University, Mazandaran, Iran. In occasion of my first thesis work, for which I spent two years working on “Genetic Diversity of Hop Stunt Viroid from Symptomatic and Asymptomatic Citrus Trees in Iran”. By this project learned useful techniques for identification and genetic diversity investigation, mainly by using specific designed primers and sequencing. For my Master's Degree Thesis I had the opportunity to work with a great research team, directed by Prof. Heshmatollah Rahimian. During this period, I became very interested in Plant metabolism and plant-microbe interaction and I realized that it was what I wanted to study in the future. 


Currently I am a researcher at Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari, Iran. During my career, I was appointed to teach and do research. I have taught several courses like Plant Bacterial and Fungal Disease, Tissue Culture Techniques, and General Microbiology for B.Sc. students and instructed several lab courses for M.Sc. students. 

RESEARCH ACTIVITIES

Medical Plant Secondary Metabolites: from In Vitro Study to Industrial Production.


Plants are the natural manufacturer of valuable materials. However, harvesting of such phytochemical from natural resources is unreliable, time consuming, laborious, land dependent, and intensively affecting by weather condition and environmental stresses. The aims of my research are focused upon using techniques in synthetic biology as a means of exploiting in vitro biosynthesis and developing the upscale industrial production. So far, I used hairy root induction by Agrobacterium rhizogenes to improve the biosynthesis of Thymol and Hypericin in Trachyspermum ammi and Hypericum perforatum respectively.




Hairy Root in Hypericum perforatum


 

Hairy Root in Trachyspermum ammi

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Medical Microbial Biosynthesis 


Microbial secondary metabolites are used as the drug active ingredient in pharmaceutical industry. Improving the biosynthesis pathway is the main focus of this project for which different techniques such as strain engineering, co culture system, and media supplementation are being applied. So far we improved lovastatin production by Aspergillus terreus using media supplementation.

Plant Growth Promoting Bacteria (PGPB) 


In this project we isolate and identify PGPB and evaluate their potential against biotic and abiotic stresses in vitro . Finally apply the selected bacteria to plants growing in field to study the mechanism of such resistance. We have obtained several pseudomonas and lactic acid bacteria species showing great antagonistic activity as well.    

Establishment of Lactic Acid Bacteria as Post Harvest Biopreservation 


Post harvest disease causing great losses annually and forcing the farmers to use pesticide to avoid it. The use of pesticide is not only failed in some cases because of pesticide resistance but also causes severe health problem for the consumers. This project conducted to address two important post harvest disease: I) Grey mold in strawberry caused by Botrytis cinerea , and II) yellow-green mold in maize caused by Aspergillus parasiticus and A. Flavus producing aflatoxin. We isolated Lactic acid bacteria from livestock gastrointestinal with the ability of control those fingy. Since they possessed probiotic properties, they are well suited to be used as bio preservation to control the postharvest disease. of are known as probiotic lactic preserve strawberry from B. Cinerea. 

A Novel Approach of Using Bio Adsorbent to Remove Heavy Metals from Vegetables


Industrial effluents and intensive use of fertilizers and pesticide over decades has increased the concentration of heavy metals in soil and water leading to contaminated agricultural products. Excessive build-up of heavy metals such as copper (Cu), cadmium (Cd), lead (Pb), nickel (Ni), and chromium (Cr) in commonly used vegetables have been widely reported. In this study we used pomegranate marc, the byproduct of Juice industry, as bio adsorbent to reduce such heavy metals from vegetable before use. 

Paper submitted for publication in refereed journals

Medium supplementation to improve lovastatin production by Aspergillus terreus. Vamenani R., Mirza Razzaza,  T., Mardani M., Javanpour M. Under review (March 2020)

A Novel Approach of Using Bio Adsorbent to Remove Heavy Metals from Vegetables. Tarlan M. R., Vamenani R., Ziarati P. Under review (April 2020)

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